Anti-MED31 antibody
| Name | Anti-MED31 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab54761 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2b |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant full length protein, corresponding to amino acids 1-132 of Human MED31 |
| Description | Mouse Monoclonal |
| Gene | MED31 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
MED31 antibody (ab54761) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human placenta.
![Overlay histogram showing HeLa cells stained with ab54761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54761, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/21057_MED31-Primary-antibodies-ab54761-1.jpg)
Overlay histogram showing HeLa cells stained with ab54761 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab54761, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Adenovirus L-E1A activates transcription through mediator complex-dependent - Adenovirus L-E1A activates transcription through mediator complex-dependent
Vijayalingam S, Chinnadurai G. J Virol. 2013 Mar;87(6):3425-34.
A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA - A conserved Mediator-CDK8 kinase module association regulates Mediator-RNA
Tsai KL, Sato S, Tomomori-Sato C, Conaway RC, Conaway JW, Asturias FJ. Nat Struct Mol Biol. 2013 May;20(5):611-9.