Anti-MEIS1 antibody - ChIP Grade
| Name | Anti-MEIS1 antibody - ChIP Grade |
|---|---|
| Supplier | Abcam |
| Catalog | ab19867 |
| Prices | $400.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P ChIPseq ChIP WB ICC/IF ICC/IF |
| Species Reactivities | Mouse, Rat, Human, Zebrafish, Dog |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human MEIS1 |
| Blocking Peptide | Human MEIS1 peptide |
| Description | Rabbit Polyclonal |
| Gene | MEIS1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml (blocked with 5% milk) + KG-1 (Human myeloid cell line) Nuclear Lysate at 15 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutionPerformed under reducing conditions.
Anti-MEIS1 antibody - ChIP Grade (ab19867) at 1 µg/ml + HEK293 Wcl Transiently Overexpressing MEIS1 at 5 µgSecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) (ab65484) at 1/3000 dilutionPerformed under reducing conditions.
IHC image of MEIS1 staining in Rat normal brain formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab19867, 0.5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
Identification and characterization of Hoxa9 binding sites in hematopoietic - Identification and characterization of Hoxa9 binding sites in hematopoietic
Huang Y, Sitwala K, Bronstein J, Sanders D, Dandekar M, Collins C, Robertson G, MacDonald J, Cezard T, Bilenky M, Thiessen N, Zhao Y, Zeng T, Hirst M, Hero A, Jones S, Hess JL. Blood. 2012 Jan 12;119(2):388-98.
Dynamic hydroxymethylation of deoxyribonucleic acid marks - Dynamic hydroxymethylation of deoxyribonucleic acid marks
Serandour AA, Avner S, Oger F, Bizot M, Percevault F, Lucchetti-Miganeh C, Palierne G, Gheeraert C, Barloy-Hubler F, Peron CL, Madigou T, Durand E, Froguel P, Staels B, Lefebvre P, Metivier R, Eeckhoute J, Salbert G. Nucleic Acids Res. 2012 Sep 1;40(17):8255-65. Epub 2012 Jun 22.
A GWAS sequence variant for platelet volume marks an alternative DNM3 promoter in - A GWAS sequence variant for platelet volume marks an alternative DNM3 promoter in
Nurnberg ST, Rendon A, Smethurst PA, Paul DS, Voss K, Thon JN, Lloyd-Jones H, Sambrook JG, Tijssen MR, Italiano JE Jr, Deloukas P, Gottgens B, Soranzo N, Ouwehand WH. Blood. 2012 Dec 6;120(24):4859-68.
Densely interconnected transcriptional circuits control cell states in human - Densely interconnected transcriptional circuits control cell states in human
Novershtern N, Subramanian A, Lawton LN, Mak RH, Haining WN, McConkey ME, Habib N, Yosef N, Chang CY, Shay T, Frampton GM, Drake AC, Leskov I, Nilsson B, Preffer F, Dombkowski D, Evans JW, Liefeld T, Smutko JS, Chen J, Friedman N, Young RA, Golub TR, Regev A, Ebert BL. Cell. 2011 Jan 21;144(2):296-309.
The role of meis1 in primitive and definitive hematopoiesis during zebrafish - The role of meis1 in primitive and definitive hematopoiesis during zebrafish
Cvejic A, Serbanovic-Canic J, Stemple DL, Ouwehand WH. Haematologica. 2011 Feb;96(2):190-8.
GSK-3 promotes conditional association of CREB and its coactivators with MEIS1 to - GSK-3 promotes conditional association of CREB and its coactivators with MEIS1 to
Wang Z, Iwasaki M, Ficara F, Lin C, Matheny C, Wong SH, Smith KS, Cleary ML. Cancer Cell. 2010 Jun 15;17(6):597-608.
The pancreatic and duodenal homeobox protein PDX-1 regulates the ductal specific - The pancreatic and duodenal homeobox protein PDX-1 regulates the ductal specific
von Burstin J, Reichert M, Wescott MP, Rustgi AK. PLoS One. 2010 Aug 19;5(8):e12311.
Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis - Comprehensive transcriptome analysis of mouse embryonic stem cell adipogenesis
Billon N, Kolde R, Reimand J, Monteiro MC, Kull M, Peterson H, Tretyakov K, Adler P, Wdziekonski B, Vilo J, Dani C. Genome Biol. 2010;11(8):R80.
Hoxa9 regulates Flt3 in lymphohematopoietic progenitors. - Hoxa9 regulates Flt3 in lymphohematopoietic progenitors.
Gwin K, Frank E, Bossou A, Medina KL. J Immunol. 2010 Dec 1;185(11):6572-83.
Gfi1 integrates progenitor versus granulocytic transcriptional programming. - Gfi1 integrates progenitor versus granulocytic transcriptional programming.
Horman SR, Velu CS, Chaubey A, Bourdeau T, Zhu J, Paul WE, Gebelein B, Grimes HL. Blood. 2009 May 28;113(22):5466-75.