Anti-Met (c-Met) antibody [EP1454Y]

• Supplier: Abcam
• Catalog: ab51067
• Prices: $404.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EP1454Y
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Human
• Antigen: Synthetic peptide corresponding to residues near the N-terminus of human Met (c-Met)
• Blocking Peptide: Met (c-Met) peptide
• Description: Rabbit Monoclonal
• Gene: MET
• Conjugate: Unconjugated

Product images

Overlay histogram showing Jurkat cells stained with ab51067 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab51067, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit Alexa Fluor® 488 (IgG, H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter.

Anti-Met (c-Met) antibody [EP1454Y] (ab51067) at 1/2000 dilution + 293 cell lysate at 10 µgSecondaryGoat anti-Rabbit HRP labeled at 1/2000 dilution

Ab51067 (1/100-1/250) staining human Met (c-Met) in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.Ab51067 (1/100-1/250) staining human Met (c-Met) in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.

ICC/IF image of ab51067 stained HepG2 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51067, 1/1000 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

ab51067 showing positive staining in Hepatocellular carcinoma tissue.

ab51067 showing positive staining in Thyroid gland carcinoma tissue.

ab51067 showing positive staining in Normal tonsil tissue.

Product References

In vivo visualization of MET tumor expression and anticalin biodistribution with - In vivo visualization of MET tumor expression and anticalin biodistribution with

Terwisscha van Scheltinga AG, Lub-de Hooge MN, Hinner MJ, Verheijen RB, Allersdorfer A, Hulsmeyer M, Nagengast WB, Schroder CP, Kosterink JG, de Vries EG, Audoly L, Olwill SA. J Nucl Med. 2014 Apr;55(4):665-71.

Lung fibroblasts accelerate wound closure in human alveolar epithelial cells - Lung fibroblasts accelerate wound closure in human alveolar epithelial cells

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Downregulation of miRNA-31 induces taxane resistance in ovarian cancer cells - Downregulation of miRNA-31 induces taxane resistance in ovarian cancer cells

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High c-MET expression is frequent but not associated with early PSA recurrence in - High c-MET expression is frequent but not associated with early PSA recurrence in

Jacobsen F, Ashtiani SN, Tennstedt P, Heinzer H, Simon R, Sauter G, Sirma H, Tsourlakis MC, Minner S, Schlomm T, Michl U. Exp Ther Med. 2013 Jan;5(1):102-106. Epub 2012 Oct 25.

3,3'-diindolylmethane rapidly and selectively inhibits hepatocyte growth - 3,3'-diindolylmethane rapidly and selectively inhibits hepatocyte growth

Nicastro HL, Firestone GL, Bjeldanes LF. J Nutr Biochem. 2013 Nov;24(11):1882-8.

Stromal micropapillary component as a novel unfavorable prognostic factor of lung - Stromal micropapillary component as a novel unfavorable prognostic factor of lung

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Co-expression of hepatocyte growth factor and c-Met predicts peritoneal - Co-expression of hepatocyte growth factor and c-Met predicts peritoneal

Toiyama Y, Yasuda H, Saigusa S, Matushita K, Fujikawa H, Tanaka K, Mohri Y, Inoue Y, Goel A, Kusunoki M. Int J Cancer. 2012 Jun 15;130(12):2912-21.

Correlation of MACC1 and MET expression in rectal cancer after neoadjuvant - Correlation of MACC1 and MET expression in rectal cancer after neoadjuvant

Kawamura M, Saigusa S, Toiyama Y, Tanaka K, Okugawa Y, Hiro J, Uchida K, Mohri Y, Inoue Y, Kusunoki M. Anticancer Res. 2012 Apr;32(4):1527-31.

Pleomorphic phenotypes of gastrointestinal stromal tumors at metastatic sites - Pleomorphic phenotypes of gastrointestinal stromal tumors at metastatic sites

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Evaluation of the frequency of putative prostate cancer stem cells in primary and - Evaluation of the frequency of putative prostate cancer stem cells in primary and

Eaton CL, Colombel M, van der Pluijm G, Cecchini M, Wetterwald A, Lippitt J, Rehman I, Hamdy F, Thalman G. Prostate. 2010 Jun 1;70(8):875-82.