Anti-Metabotropic Glutamate Receptor 5 antibody
| Name | Anti-Metabotropic Glutamate Receptor 5 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab53090 |
| Prices | $398.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-F ICC/IF ICC/IF IHC-P ELISA WB IHC-F |
| Species Reactivities | Mouse, Rat, Chicken, Human |
| Antigen | N-terminal synthetic peptide derived from human Metabotropic Glutamate Receptor 5 |
| Description | Rabbit Polyclonal |
| Gene | GRM5 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab53090 staining Metabotropic Glutamate Receptor 5 (red) in rat hippocampla neuron by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 5% serum for 20 minutes at 22°C. Samples were incubated with primary antibody (1/200) for 24 hours at 22°C. An Alexa Fluor® 594-conjugated chicken anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody. Cyan - pre-synaptic marker Bassoon.See Abreview
All lanes : Anti-Metabotropic Glutamate Receptor 5 antibody (ab53090) at 1/300 dilutionLane 1 : Mouse brain extractsLane 2 : Mouse brain extracts with blocking peptide
Immunohistochemical analysis of paraffin embedded human brain tissue using ab53090 at 1/50 dilution, in the presence (right) and absence (left) of blocking peptide.Immunohistochemical analysis of paraffin embedded human brain tissue using ab53090 at 1/50 dilution, in the presence (right) and absence (left) of blocking peptide.
ab53090 staining Metabotropic Glutamate Receptor 5 in rat brain tissue sections by Immunohistochemistry (Free Floating).Animals were perfused fixed with 4% paraformaldehyde with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were performed using ab53090 at a 1/300 dilution. The secondary used was an Alexa Fluor 488 conjugated goat anti-rabbit used at a 1/1000 dilution.ab53090 staining Metabotropic Glutamate Receptor 5 in rat brain tissue sections by Immunohistochemistry (Free Floating).Animals were perfused fixed with 4% paraformaldehyde with 15% of a solution of saturated picric acid, in phosphate buffer 0.1M. Following postfixation in the same fixative overnight, the brains were cryoprotected in sucrose 30% overnight. Brains were then cut using a cryostat and the immunostainings were perform
Product References
Visual sensorial impairments in neurodevelopmental disorders: evidence for a - Visual sensorial impairments in neurodevelopmental disorders: evidence for a
Rossignol R, Ranchon-Cole I, Paris A, Herzine A, Perche A, Laurenceau D, Bertrand P, Cercy C, Pichon J, Mortaud S, Briault S, Menuet A, Perche O. PLoS One. 2014 Aug 25;9(8):e105996.
Amyloid beta deregulates astroglial mGluR5-mediated calcium signaling via - Amyloid beta deregulates astroglial mGluR5-mediated calcium signaling via
Lim D, Iyer A, Ronco V, Grolla AA, Canonico PL, Aronica E, Genazzani AA. Glia. 2013 Jul;61(7):1134-45.
mRNA and protein expression for novel GABAA receptors theta and rho2 are altered - mRNA and protein expression for novel GABAA receptors theta and rho2 are altered
Fatemi SH, Folsom TD, Rooney RJ, Thuras PD. Transl Psychiatry. 2013 Jun 18;3:e271.
Excitatory glutamate is essential for development and maintenance of the - Excitatory glutamate is essential for development and maintenance of the
Woo SH, Baba Y, Franco AM, Lumpkin EA, Owens DM. Development. 2012 Feb;139(4):740-8.
Changes in midbrain pain receptor expression, gait and behavioral sensitivity in - Changes in midbrain pain receptor expression, gait and behavioral sensitivity in
Hwang PY, Allen KD, Shamji MF, Jing L, Mata BA, Gabr MA, Huebner JL, Kraus VB, Richardson WJ, Setton LA. Open Orthop J. 2012;6:383-91.
Incentive learning underlying cocaine-seeking requires mGluR5 receptors located - Incentive learning underlying cocaine-seeking requires mGluR5 receptors located
Novak M, Halbout B, O'Connor EC, Rodriguez Parkitna J, Su T, Chai M, Crombag HS, Bilbao A, Spanagel R, Stephens DN, Schutz G, Engblom D. J Neurosci. 2010 Sep 8;30(36):11973-82.
Activation of metabotropic glutamate receptor 5 modulates microglial reactivity - Activation of metabotropic glutamate receptor 5 modulates microglial reactivity
Loane DJ, Stoica BA, Pajoohesh-Ganji A, Byrnes KR, Faden AI. J Biol Chem. 2009 Jun 5;284(23):15629-39.