All lanes : Anti-Methionyl tRNA synthetase antibody (ab157119) at 0.4 µg/mlLane 1 : 293T whole cell lysateLane 2 : HeLa whole cell lysateLane 3 : Jurkat whole cell lysateLane 4 : Mouse TCMK1 whole cell lysateLane 5 : Mouse NIH3T3 whole cell lysateLysates/proteins at 50 µg per lane.developed using the ECL technique
Detection of Methionyl tRNA synthetase by Western Blot of Immunprecipitate. ab157119 at 1µg/ml labeling Methionyl tRNA synthetase in 293T whole cell lysate immunoprecipitated using ab157119 at 6µg/mg lysate (1 mg/IP; 20% of IP loaded/lane). Detection: Chemiluminescence with exposure time of 30 seconds.