Anti-MFF antibody
| Name | Anti-MFF antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab81127 |
| Prices | $378.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB |
| Species Reactivities | Rat, Human, Zebrafish, Mouse, Rabbit, Horse, Chicken, Guinea Pig, Bovine, Cat, Dog |
| Antigen | Synthetic peptide corresponding to a region within C terminal amino acids 287-336 (LTVVDAASLR RQIIKLNRRL QLLEEENKER AKREMVMYSI TVAFWLLNSW) of Human MFF (NP_064579) |
| Description | Rabbit Polyclonal |
| Gene | MFF |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Anti-MFF antibody (ab81127) at 1 µg/ml (in 5% skim milk / PBS buffer) + fetal muscle lysate at 10 µgSecondaryHRP conjugated anti-Rabbit IgG at 1/50000 dilution
All lanes : Anti-MFF antibody (ab81127) at 1 µg/mlLane 1 : MarkerLane 2 : Zebrafish brain homogenate at 20 µgLane 3 : Zebrafish heart homogenate at 20 µgLane 4 : Zebrafish liver homogenate at 20 µgSecondaryGoat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab81127 stained A549 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab81127 at 5µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- rabbit (ab96899) IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.