Anti-MIOX antibody [EPR17172]

• Supplier: Abcam
• Catalog: ab199729
• Prices: $347.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR17172
• Applications: WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MIOX aa 1-100
• Description: Rabbit Monoclonal
• Gene: MIOX
• Conjugate: Unconjugated

Product images

All lanes : Anti-MIOX antibody [EPR17172] (ab199729) at 1/5000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal heart lysateLane 3 : Human fetal spleen lysateLane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution

All lanes : Anti-MIOX antibody [EPR17172] (ab199729) at 1/5000 dilutionLane 1 : Mouse kidney lysateLane 2 : Mouse brain lysateLane 3 : Mouse heart lysateLane 4 : Mouse spleen lysateLane 5 : RAW 264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysateLane 6 : NIH/3T3 (Mouse embyro fibroblast cells) whole cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

MIOX was immunoprecipitated from 1mg of Mouse kidney whole cell lysate with ab199729 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab199729 at 1/2000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Mouse kidney whole cell lysate 10 µg (Input). Lane 2: ab199729 IP in Mouse kidney whole cell lysate. Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199729 in Mouse kidney whole cell lysate.Blocking and dilution buffer and concentration: 5% NFDM/TBST.