All lanes : Anti-MIOX antibody [EPR17173] (ab198997) at 1/2000 dilutionLane 1 : Human fetal kidney lysateLane 2 : Human fetal heart lysateLysates/proteins at 10 µg per lane.SecondaryAnti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
MIOX was immunoprecipitated from 1mg of Human fetal kidney whole cell extract with ab198997 at 1/150 dilution. Western blot was performed using ab198997 at 1/1000 dilution. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.Lane 1: Human fetal kidney whole cell extract 10µg (Input).Lane 2: ab198997 IP in Human fetal kidney whole cell extract.Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab198997 in Human fetal kidney whole cell extract.Blocking and dilution buffer and concentration: 5% NFDM/TBST.