Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)]

• Supplier: Abcam
• Catalog: ab133789
• Prices: $387.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR9111(B)
• Applications: WB IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Human
• Antigen: A synthetic peptide corresponding to residues at the C-terminus of Human Mitochondrial ribosomal protein L11 (UniProt ID: Q9Y3B7)
• Description: Rabbit Monoclonal
• Gene: MRPL11
• Conjugate: Unconjugated

Product images

Immunohistochemical analysis using ab133789(1/250-1/500 dilution) of mouse liver tissue showing staining in cytoplasm.

Immunohistochemical analysis using ab133789(1/250-1/500 dilution) of mouse colon tissue showing staining in cytoplasm.

Overlay histogram showing HepG2 cells stained with ab133789 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab133789, 1/1000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-Mitochondrial ribosomal protein L11 antibody [EPR9111(B)] (ab133789) at 1/1000 dilutionLane 1 : HeLa cell lysateLane 2 : HepG2 cell lysateLane 3 : LNCaP cell lysateLysates/proteins at 10 µg per lane.SecondaryHRP-labelled Goat anti-Rabbit at 1/2000 dilution

Immunofluorescent staining of Mitochondrial ribosomal protein L11 in HEPG2 cells using ab133789 at 1/250 dilution

Immunohistochemical analysis of paraffin-embedded human kidney tissue labelling Mitochondrial ribosomal protein L11 using ab133789 at 1/250 dilution

Immunohistochemical analysis of paraffin-embedded human colon tissue labelling Mitochondrial ribosomal protein L11 using ab133789 at 1/250 dilution

Immunohistochemical analysis using ab133789 showing positive staining in Normal colon tissue.

Immunohistochemical analysis using ab133789 showing positive staining in Normal heart tissue.

Immunohistochemical analysis using ab133789 showing positive staining in Normal lung tissue.

Immunohistochemical analysis using ab133789 showing positive staining in Normal stomach tissue.

Equilibrium disassociation constant (KD)Learn more about KD Click here to learn more about KD