All lanes : Anti-Mkln1 antibody [EPR13519] (ab178403) at 1/1000 dilutionLane 1 : Jurkat cell lysateLane 2 : A431 cell lysateLane 3 : Y79 cell lysateLane 4 : 293T cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilutiondeveloped using the ECL technique
Flow cytometry analysis of permeabilized Jurkat cells labeling Mkln1 (red) with ab178403 at a 1/100 dilution, or negative control rabbit IgG (green)
Western blot analysis on immunoprecipitation pellet from 293T cell lysate labeling Mkln1 with ab178403 at 1/10 dilution.