Anti-MLH1 antibody [EPR3894]

• Supplier: Abcam
• Catalog: ab92312
• Prices: $403.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR3894
• Applications: WB IP IHC-P FC ICC/IF ICC/IF
• Species Reactivities: Mouse, Rat, Human
• Antigen: A synthetic peptide corresponding to residues in Human MLH1 (UniProt ID: P40692)
• Description: Rabbit Monoclonal
• Gene: MLH1
• Conjugate: Unconjugated

Product images

All lanes : Anti-MLH1 antibody [EPR3894] (ab92312) at 1/10000 dilutionLane 1 : 293T cell lysateLane 2 : HeLa cell lysateLane 3 : A431 cell lysateLane 4 : SW480 cell lysateLysates/proteins at 10 µg per lane.Secondarygoat anti-rabbit HRP at 1/2000 dilution

ab92312 at 1/100 dilution staining MLH1 in Human colonic adenocarcinoma by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

ab92312 (1/200) staining MLH1 in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.5% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to abreview.See Abreview

Overlay histogram showing HeLa cells stained with ab92312 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab92312, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

ab92312 staining MLH1 in Human colorectal (top) and gastric tissue (bottom) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).

ab92312 at 1/100 dilution staining MLH1 in Human tonsil by Immunohistochemistry, Paraffin-embedded tissue. The use of an HRP/AP polymerized antibody is recommended for a secondary antibody.

Product References

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Nelson GS, Pink A, Lee S, Han G, Morris D, Ogilvie T, Duggan MA, Kobel M. Gynecol Oncol. 2013 Nov;131(2):309-14.

Spreading of Alu methylation to the promoter of the MLH1 gene in gastrointestinal - Spreading of Alu methylation to the promoter of the MLH1 gene in gastrointestinal

Wang X, Fan J, Liu D, Fu S, Ingvarsson S, Chen H. PLoS One. 2011;6(10):e25913.