Anti-MMP10 antibody [5E4]
| Name | Anti-MMP10 antibody [5E4] |
|---|---|
| Supplier | Abcam |
| Catalog | ab49473 |
| Prices | $378.00 |
| Sizes | 200 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | 5E4 |
| Applications | IHC-P ICC/IF ICC/IF |
| Species Reactivities | Human |
| Antigen | Prokaryotic recombinant fusion protein corresponding to amino acids 342-476 of the human MMP10 molecule |
| Description | Mouse Monoclonal |
| Gene | MMP10 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab49473 stained HeLa cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal Goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab49473, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 Goat anti-Mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.