Anti-MMP14 antibody
| Name | Anti-MMP14 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab3644 |
| Prices | $391.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | FC IHC-P IHC-F WB ICC/IF ICC/IF ICC/IF IP |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: REVPYAYIREGHEK , corresponding to amino acids 160-173 of Human MMP14 Run BLAST with Run BLAST with |
| Description | Rabbit Polyclonal |
| Gene | MMP14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MMP14 antibody (ab3644) at 1/500 dilutionLane 1 : 15ug MCF-7 whole cell lysateLane 2 : 15ug MSTO-2H11 whole cell lysateLane 3 : 15ug Malme-3M whole cell lysateLane 4 : 15ug Calu-1 whole cell lysateLane 5 : 15ug Sk-Lu-1 whole cell lysateSecondaryPeroxidase-conjugated affinipure goat anti-rabbitdeveloped using the ECL techniquePerformed under reducing conditions.Observed band size : 54,66 kDa (why is the actual band size different from the predicted?)Additional bands at : 20 kDa (possible non-specific binding),98 kDa (possible non-specific binding).Exposure time : 30 secondsSee Abreview
ICC/IF image of ab3644 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab3644, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
IHC image of ab3644 staining in Hu Colon formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab3644, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
Product References
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Neill T, Painter H, Buraschi S, Owens RT, Lisanti MP, Schaefer L, Iozzo RV. J Biol Chem. 2012 Feb 17;287(8):5492-506.
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Ling Poon S, Lau MT, Hammond GL, Leung PC. Endocrinology. 2011 Mar;152(3):764-72.
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Decorin antagonizes the angiogenic network: concurrent inhibition of Met, hypoxia - Decorin antagonizes the angiogenic network: concurrent inhibition of Met, hypoxia
Neill T, Painter H, Buraschi S, Owens RT, Lisanti MP, Schaefer L, Iozzo RV. J Biol Chem. 2012 Feb 17;287(8):5492-506.
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Kean MJ, Williams KC, Skalski M, Myers D, Burtnik A, Foster D, Coppolino MG. J Cell Sci. 2009 Nov 15;122(Pt 22):4089-98.