![MMP14 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MMP14 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73879.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 65kDa; MMP14](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23368_ab73879-171827-IPV018ab7387920mMod.jpg)
MMP14 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MMP14 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-).The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70°C; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab73879.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 65kDa; MMP14
Anti-MMP14 antibody (ab73879) at 1 µg/ml + Hela cell lysate at 50 µgSecondaryHRP-conjugated Goat anti-rabbit IgG at 3000 µg
Immunohistochemical analysis of frozen sections Rat kindey tissue using MMP14 antibody ab73879 at 1 - 2 µg/ml.
Immunohistochemical analysis of paraffin-embedded sections Rat small intestine using MMP14 antibody ab73879 at 1 - 2 µg/ml. A Biotin-conjugated Goat anti-rabbit IgG was used as secondary at 1/200 dilution.