Anti-MMP14 antibody [EP1264Y]
| Name | Anti-MMP14 antibody [EP1264Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab51074 |
| Prices | $401.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1264Y |
| Applications | IHC-P ICC/IF ICC/IF FC IP WB |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues in human MMP14 |
| Blocking Peptide | MMP14 peptide |
| Description | Rabbit Monoclonal |
| Gene | MMP14 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
![Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution + Human spleen lysate SecondaryGoat anti-Rabbit HRP labeled. at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23380_ab51074_2.gif)
Anti-MMP14 antibody [EP1264Y] (ab51074) at 1/2000 dilution + Human spleen lysate SecondaryGoat anti-Rabbit HRP labeled. at 1/2000 dilution
Ab51074 (1:100) staining human MMP14 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.Ab51074 (1:100) staining human MMP14 in human breast carcinoma tissue by immunohistochemistry using paraffin embedded tissue.
ab51074 at 1/500 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step (in Tris/EDTA) was performed. The tissue was then blocked with serum and incubated with the primary antibody. A biotinylated donkey anti-rabbit IgG was used as the secondary.See Abreview
Overlay histogram showing HT1080 cells stained with ab51074 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab51074, 1µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was a goat anti-rabbit DyLight® 488 (IgG, H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit monoclonal IgG (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HT1080 cells fixed with methanol (5 min) used under the same conditions.Please note that Abcam does not have data for use of this antibody on non-fixed cells. We welcome any customer feedback.
Product References
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Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK - Transmembrane/cytoplasmic, rather than catalytic, domains of Mmp14 signal to MAPK
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