All lanes : Anti-MMP16 antibody - Cytoplasmic domain (ab38967) at 1/1000 dilutionLane 1 : Whole cell lysate prepared from human prostate carcinoma 22Rv-1 cells (1:8) (treated with SF)Lane 2 : Whole cell lysate prepared from human prostate carcinoma 22Rv-1 cells (1:8) (treated with TPA)Lane 3 : Whole cell lysate prepared from human epithelial carcinoma A-431 cells (1:5) (treated with SF)Lane 4 : Whole cell lysate prepared from human epithelial carcinoma A-431 cells (1:5) (treated with TPA)Lysates/proteins at 15 µl per lane.