Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling MMP22 with ab181118 at 1/100 followed by fluorescence labelled Goat anti-rabbit IgG at 1/200 and counterstained with Dapi.
Immunohistochemical analysis of paraffin embedded Human testis tissue labeling MMP22 with ab181118 at 1/250.
All lanes : Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilutionLane 1 : Calu 3 cell lysateLane 2 : SK OV 3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated antibody at 1/1000 dilution
Anti-MMP22 antibody [EPR7769] (ab181118) at 1/1000 dilution + Human fetal brain tissue lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab136636) at 1/500 dilution