Anti-MMP25 antibody
| Name | Anti-MMP25 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39031 |
| Prices | $380.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB IP |
| Species Reactivities | Mouse, Human |
| Antigen | Synthetic peptide based on hinge region of MMP25 (Human) |
| Description | Rabbit Polyclonal |
| Gene | MMP25 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab39031 staining MMP25 in moderately differentiated gland-forming invasive human colorectal carcinoma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Four-micron-thick sections of the arrays were deparaffinized, and antigen retrieval was performed by microwaving slides in 7.5 mm sodium citrate buffer, pH 6.0. After a brief rinse in Tris-buffered saline (TBS), pH 8.0, endogenous peroxidase activity and non-specific background staining were blocked by incubating for 30 minutes in 3% hydrogen peroxide in methanol followed by 30 minutes in 0.3% bovine serum albumin in TBS. Slides were rinsed for 2 minutes each in TBS, TBS containing 0.01% Triton X-100, and TBS followed by incubation for 1 hour at room temperature with ab39031 at a 1/400 dilution. Slides were then rinsed in TBS and incubated for 30 minutes with goat anti-rabbit IgG conjugated to a horseradish peroxidase-labeled polymer. This incubation was followed by additional TBS rinses, visualization with
All lanes : Anti-MMP25 antibody (ab39031)Lane 1 : Secreted fraction of HT-29 cells treated with DMSO Lane 2 : Secreted fraction of HT-29 cells treated with 100 µM sulindac sulfideSecondaryHRP-conjugated anti-rabbit IgGdeveloped using the ECL technique
Product References
Biochemical characterization and N-terminomics analysis of leukolysin, the - Biochemical characterization and N-terminomics analysis of leukolysin, the
Starr AE, Bellac CL, Dufour A, Goebeler V, Overall CM. J Biol Chem. 2012 Apr 13;287(16):13382-95.
Effect of sulindac sulfide on metallohydrolases in the human colon cancer cell - Effect of sulindac sulfide on metallohydrolases in the human colon cancer cell
Guillen-Ahlers H, Tan J, Castellino FJ, Ploplis VA. PLoS One. 2011;6(10):e25725.
MMP25 (MT6-MMP) is highly expressed in human colon cancer, promotes tumor growth, - MMP25 (MT6-MMP) is highly expressed in human colon cancer, promotes tumor growth,
Sun Q, Weber CR, Sohail A, Bernardo MM, Toth M, Zhao H, Turner JR, Fridman R. J Biol Chem. 2007 Jul 27;282(30):21998-2010. Epub 2007 May 18.