All lanes : Anti-MMP3 antibody (ab63853) at 1 µg/mlLane 1 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lane 2 : Liver (Human) Tissue Lysate - adult normal tissue (ab29889)Lane 3 : Lung (Human) Tissue LysateLysates/proteins at 10 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
ab63853 staining MMP3 in Human breast carcinoma tissue sections by IHC-P (formaldehyde fixed paraffin embedded sections). Tissue was fixed with formaldehyde and blocked by 5 minutes of peroxidase block then 10 minutes of protein block. Antigen retrieval was by heat mediation in Retrieval solution. Samples were incubated with primary antibody (1/50) for 45 minutes at 20°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.See Abreview