Anti-MMP7 antibody

• Supplier: Abcam
• Catalog: ab4044
• Prices: $398.00
• Sizes: 500 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: WB IHC-P IHC-F ICC/IF ICC/IF ICC/IF
• Species Reactivities: Mouse, Human
• Antigen: Synthetic peptide corresponding to Human MMP7 aa 1-267
• Description: Rabbit Polyclonal
• Gene: MMP7
• Conjugate: Unconjugated

Product images

ab4044 staining MMP7 in Human stomach tissue sections by IHC-Fr (Immunohistochemistry - Froxen sections). Tissue samples were fixed with acetone and blocked with 5% serum for 1 hour at 25°C. Samples were incubated with primary antibody 1/50 in blocking buffer for 1 hour at 25°C. An undiluted HRP-conjugated Goat polyclonal to rabbit IgG was used as secondary antibody.See Abreview

ab4044 at 1/50 dilution staining MMP7 in human stomach tissue by immunohistochemistry (formalin/PFA-fixed paraffin-embedded sections). Sections were formaldehyde fixed prior to blocking in 5% serum for 1 hour at 23°C and then incubated with ab4044 for 1 hour at 23°C. A HRP conjugated goat polyclonal to rabbit Ig was used as the secondary antibody.See Abreview

ab4044 staining MMP7 in Human AGS Gastric Carcinoma cells by immunocytochemistry/ Immunofluorescence. The cells were formaldehyde fixed, permeabilized in TBS (0.025% Triton-X 100) and blocked in 5% serum for 1 hour at 23°C. The primary was diluted 1/50 and incubated with sample for 1 hour at 23°C. An Alexa Fluor® 568 conjugated Goat polyclonal to Rabbit IgG at 1/500 dilution, was used as secondary antibody. The primary antibody shows red staining of MMP7 in image whilst beta actin was stained green with Alexa Fluor® 488.See Abreview

Anti-MMP7 antibody (ab4044) at 1/1000 dilution (in blocking buffer for 16 hours at 4°C) + Whole cell lysate of Human AGS gastric carcinoma cells at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab6721) at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 1 minuteThis image is courtesy of an anonymous AbreviewSee Abreview

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human non-small-cell lung cancer tissue staining MMP7 with ab4044. Antigen retrieval was performed by placing the sections in 0.01 M citrate buffer (pH 6) and heating in a microwave oven. Samples were incubated with primary antibody (1/15) overnight at 4°C. Staining was detected using an IHC substrate kit.

Product References

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