Anti-MMP9 antibody

• Supplier: Abcam
• Catalog: ab38898
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-P IHC-F WB IP ELISA ICC/IF ICC/IF ICC/IF IHC-F
• Species Reactivities: Mouse, Rat, Dog, Human
• Antigen: Full length protein corresponding to Mouse MMP9
• Description: Rabbit Polyclonal
• Gene: MMP9
• Conjugate: Unconjugated

Product images

Anti-MMP9 antibody (ab38898) + Human MMP9Observed band size : 88,92 kDa (why is the actual band size different from the predicted?)Ab38898 detects a band at 92 Kd (pro-form) and a band at 88 Kd (active form). Mouse MMP9 is slightly larger than human MMP9, and the antibody detects a band at about 105 Kd. It is recommend to concentrate samples by Gelatin-agarose affinity chromatography prior to Western blot usage. A recommended starting concentration for Western blots is 1:1000 when using colorimetric substrates such as BCIP/NBT, and 1:5000 for chemiluminescent substrates. Higher concentration of antibody may be needed for non-human samples.

All lanes : Anti-MMP9 antibody (ab38898) at 1/1000 dilutionLane 1 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperatureLane 2 : Human lung tissue lysate at 100 µg with 10% Milk for 1 hour at room temperatureLane 3 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperatureLane 4 : MMP-9 KO mice tissue lysates with 10% Milk for 1 hour at room temperatureSecondaryHRP-conjugated donkey anti-rabbit polyclonal at 1/1000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.This image is courtesy of an anonymous AbreviewSpecific observed bands 95-100 kDaSee Abreview

ab38898 at a 1/1000 dilution staining mouse heart tissue by Immunohistochemistry (Frozen sections). The tissue was removed from a mouse, rinsed in PBS and slowly frozen in supercooled isopentane. 14um sections were made using a cryostat. The sections were acetone fixed and blocked in 2% BSA prior to incubation with the MMP9 antibody. A Cy3 conjugated goat anti-rabbit antibody (ab6939) was used as the secondary antibody. In the image: red staining = MMP9, blue staining = nuclei, green = gelatinase activity.

ab38898 staining MMP9 in Mouse Pancreatic carcinoma tissue sections by IHC-P (formaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 1% BSA for 1 hour at room temperature. Antigen retrieval was by heat mediation in citric acid (pH6). Samples were incubated with primary antibody (1/100) in 1% Aurion BSA for 12 hours. An HRP-conjugated Donkey polyclonal to rabbit IgG (1/100) was used as secondary antibody.See Abreview

ab38898 staining MMP9 in 6 month-old transgenic zebrafish pancreas (Ihha overexpression) by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections). Sections were incubated with primary antibody (1/500) and HRP-conjugated secondary antibody colored using DAB solution. Slides were counterstained with hematoxylin.

Product References

IL-6-mediated induction of matrix metalloproteinase-9 is modulated by - IL-6-mediated induction of matrix metalloproteinase-9 is modulated by

Kothari P, Pestana R, Mesraoua R, Elchaki R, Khan KM, Dannenberg AJ, Falcone DJ. J Immunol. 2014 Jan 1;192(1):349-57.

Molecular changes in bone marrow, tumor and serum after conductive ablation of - Molecular changes in bone marrow, tumor and serum after conductive ablation of

Przybyla BD, Shafirstein G, Vishal SJ, Dennis RA, Griffin RJ. Int J Oncol. 2014 Feb;44(2):600-8.

Acute lymphoid leukemia cells with greater stem cell antigen-1 (Ly6a/Sca-1) - Acute lymphoid leukemia cells with greater stem cell antigen-1 (Ly6a/Sca-1)

Hsu YC, Mildenstein K, Hunter K, Tkachenko O, Mullen CA. PLoS One. 2014 Feb 20;9(2):e88966.

Aldose reductase inhibition prevents endotoxin-induced inflammatory responses in - Aldose reductase inhibition prevents endotoxin-induced inflammatory responses in

Chang KC, Ponder J, Labarbera DV, Petrash JM. Invest Ophthalmol Vis Sci. 2014 May 2;55(5):2853-61.

Combination of bexarotene and the retinoid CD1530 reduces murine oral-cavity - Combination of bexarotene and the retinoid CD1530 reduces murine oral-cavity

Tang XH, Osei-Sarfo K, Urvalek AM, Zhang T, Scognamiglio T, Gudas LJ. Proc Natl Acad Sci U S A. 2014 Jun 17;111(24):8907-12. doi:

Effects of omega3- and omega6-polyunsaturated fatty acids on RANKL-induced - Effects of omega3- and omega6-polyunsaturated fatty acids on RANKL-induced

Boeyens JC, Deepak V, Chua WH, Kruger MC, Joubert AM, Coetzee M. Nutrients. 2014 Jul 9;6(7):2584-601.

Molecular basis for MMP9 induction and disruption of epithelial cell-cell - Molecular basis for MMP9 induction and disruption of epithelial cell-cell

Mauris J, Woodward AM, Cao Z, Panjwani N, Argueso P. J Cell Sci. 2014 Jul 15;127(Pt 14):3141-8.

Rac1 regulates platelet shedding of CD40L in abdominal sepsis. - Rac1 regulates platelet shedding of CD40L in abdominal sepsis.

Hwaiz R, Rahman M, Zhang E, Thorlacius H. Lab Invest. 2014 Sep;94(9):1054-63.

MMP-9 expression varies according to molecular subtypes of breast cancer. - MMP-9 expression varies according to molecular subtypes of breast cancer.

Yousef EM, Tahir MR, St-Pierre Y, Gaboury LA. BMC Cancer. 2014 Aug 23;14:609.

Heat shock protein 60 overexpression is associated with the progression and - Heat shock protein 60 overexpression is associated with the progression and

Li XS, Xu Q, Fu XY, Luo WS. PLoS One. 2014 Sep 10;9(9):e107507.