![Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (unpurified) + U937 cell lysate at 10 µgSecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23688_ab137867-238898-ab137867upwb.jpg)
Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (unpurified) + U937 cell lysate at 10 µgSecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution
![Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (purified) + U937 cell lysate at 10 µgSecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/23689_ab137867-238899-ab137867pwb.jpg)
Anti-MMP9 antibody [EP1255Y] (ab137867) at 1/1000 dilution (purified) + U937 cell lysate at 10 µgSecondaryPeroxidase conjugated goat anti-rabbit IgG (H+L) at 1/1000 dilution

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human tonsil tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling MMP9 with unpurified ab137867 at 1/800. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling MMP9 with purified ab137867 at 1/1000. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. A prediluted HRP-polymer conjugated anti-rabbit IgG was used as the secondary antibody. Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat spleen tissue labelling MMP9 with unpurified ab137867 at 1/1000. This results in staining of macrophages.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat colon tissue labelling MMP9 with unpurified ab137867 at 1/1000. This results in staining of macrophages.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse lung tissue labelling MMP9 with unpurified ab137867 at 1/2000. This results in staining of macrophages.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse spleen tissue labelling MMP9 with unpurified ab137867 at 1/2000. This results in staining of macrophages.

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human lymphoma tissue labelling MMP9 with unpurified ab137867 at 1/100.

Unpurified ab137867 staining MMP9 in 12µm dog atria tissue sections by Immunohistochemistry (IHC-Fr - frozen sections). Tissue was fixed with 4% paraformaldehyde, permeabilized and blocked with PBS + 0.3% Triton X-100 + 10mg/mL BSA + 5% normal goat serum for 1 hour at 25°C. Samples were incubated with primary antibody (1/100 in PBS + 10mg/mL BSA + 5% normal goat serum) for 6 hours at 25°C. A Cy2®-conjugated goat anti-rabbit IgG polyclonal (1/200) was used as the secondary antibody.See Abreview