Anti-MPG antibody
| Name | Anti-MPG antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55461 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | WB IHC-P FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: MPARSGAQFC RRMGQKKQRP ARAGQPHSSS DAAQAPAEQP HSSSDAAQAP CPRERCLGPP TTPGPYRSIY FSSPKGHLTR LGLEFFDQPA , corresponding to amino acids 1-90 of Human MPG Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | MPG |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
MPG antibody (ab55461) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human kidney.
ab55461 staining MPG in human mammary tissue by Immunohistochemistry (paraffin embedded sections).Paraffin-embedded blocks were sectioned and mounted on frost-free slides. The 3-10 µm sections were deparaffinized in xylene and rehydrated through a series of graded alcohols. Slides were washed with 1× PBS and endogenous peroxidases were blocked with 1.5% hydrogen peroxide in 1× PBS for 20 minutes at 25°C. After three 5 minutes washes in 1× PBS, slides were incubated in blocking solution (1× PBS with 0.1% Triton X-100, 3% bovine serum albumin) with 5% normal donkey serum for 10 minutes at 25°C. Control (no primary antibody) and experimental slides were incubated overnight at 4°C, respectively, in blocking solution alone or blocking solution with ab55461 at 1/600 dilution. Biotin-conjugated secondary antibody 1/200 was added and slides were incubated at 25°C for 30 minutes and then washed three times with 1× PBS. The ABC Peroxidase Staining kit (1:100 dilution of each Reagent A and B in 1
Anti-MPG antibody (ab55461) at 1 µg/ml + Human MPG full length protein (ab109955) at 0.1 µgSecondaryGoat Anti-Mouse IgG H&L (HRP) preadsorbed (ab97040) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 10 seconds
Overlay histogram showing HeLa cells stained with ab55461 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55461, 1/100 dilution) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Immunohistochemical analysis of Human gliomas, staining MPG with ab55461. Formalin fixed, paraffin-embedded tissue was blocked with 5% normal horse serum and incubated with primary antibody (1/500) overnight at 4°C. Staining was detected using DAB.
Product References
An inverse switch in DNA base excision and strand break repair contributes to - An inverse switch in DNA base excision and strand break repair contributes to
Sousa MM, Zub KA, Aas PA, Hanssen-Bauer A, Demirovic A, Sarno A, Tian E, Liabakk NB, Slupphaug G. PLoS One. 2013;8(2):e55493.
Aberrant expression of N-methylpurine-DNA glycosylase influences patient survival - Aberrant expression of N-methylpurine-DNA glycosylase influences patient survival
Liu C, Tu Y, Yuan J, Mao X, He S, Wang L, Fu G, Zong J, Zhang Y. J Biomed Biotechnol. 2012;2012:760679.
The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic - The mammalian proteins MMS19, MIP18, and ANT2 are involved in cytoplasmic
van Wietmarschen N, Moradian A, Morin GB, Lansdorp PM, Uringa EJ. J Biol Chem. 2012 Dec 21;287(52):43351-8.
Immunohistochemical analysis of oxidative stress and DNA repair proteins in - Immunohistochemical analysis of oxidative stress and DNA repair proteins in
Curtis CD, Thorngren DL, Nardulli AM. BMC Cancer. 2010 Jan 11;10:9.