All lanes : Anti-MPG antibody [EPR10959(B)] (ab155092) at 1/10000 dilutionLane 1 : HeLa cell lysateLane 2 : 293T cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat anti-rabbit HRP at 1/2000 dilution
Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labeling MPG with ab155092 at 1/50 dilution.
Immunohistochemical analysis of paraffin-embedded Human tesis tissue labeling MPG with ab155092 at 1/50 dilution.
Immunohistochemical analysis of paraffin embedded Human lung adenocarcinoma tissue using ab155092 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human normal colon tissue using ab155092 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human cervical carcinoma tissue using ab155092 showing +ve staining.
Immunohistochemical analysis of paraffin embedded Human normal breast tissue using ab155092 showing +ve staining.
ab155092 staining MPG in human primary fibroblasts by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde, permeabilized with 0.2% Triton X-100 and blocked with 2% BSA for 1 hour at room temperature. Samples were incubated with primary antibody (1/500 in PBS + 2% BSA) for 14 hours at 4°C. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.See Abreview
ab155092 (1/500) staining MPG in HeLa cells (green). Cells were fixed in paraformaldehyde, permeabilized with 0.5%Triton X-100/PBS and counterstained with DAPI in order to highlight the nucleus (red). For further experimental details please refer to Abreview.See Abreview