All lanes : Anti-MPP3 antibody - C-terminal (ab171369) at 1/100 dilutionLane 1 : K562 cell line lysateLane 2 : NCI-H292 cell line lysateLysates/proteins at 35 µg per lane.
Immunofluorescent analysis of MDA-MB23 cells labeling MPP3 with ab171369 at 1/10 dilution followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green) and DAPI staining.
Flow Cytometric analysis of K562 cells labeling MPP3 with ab171369 at 1/10 dilution (right histogram) compared to a negative control cell (left histogram). FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.