Anti-MRP8 + MRP14 antibody [MAC 387]
| Name | Anti-MRP8 + MRP14 antibody [MAC 387] |
|---|---|
| Supplier | Abcam |
| Catalog | ab80708 |
| Prices | $378.00 |
| Sizes | 100 µl |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone | MAC 387 |
| Applications | FC IHC-P |
| Species Reactivities | Mouse, Rat, Guinea Pig, Cat, Dog, Human, Pig, Monkey, Monkey |
| Antigen | Affinity purified monocyte membrane preparation |
| Description | Mouse Monoclonal |
| Gene | S100A8 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Formalin-fixed, paraffin-embedded human tonsil stained with ab80708 at a 1/1000 dilution using peroxidase-conjugate and AEC chromogen. Note cytoplasmic staining of macrophages.
![Overlay histogram showing THP1 cells stained with ab80708 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80708, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/24516_MRP8-MRP14-Primary-antibodies-ab80708-6.jpg)
Overlay histogram showing THP1 cells stained with ab80708 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab80708, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in THP1 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
Acute kidney graft rejection. A morphological and immunohistological study on - Acute kidney graft rejection. A morphological and immunohistological study on
Andersen CB, Ladefoged SD, Larsen S. APMIS. 1994 Jan;102(1):23-37.
Expression of the myelomonocytic antigens CD36 and L1 by keratinocytes in - Expression of the myelomonocytic antigens CD36 and L1 by keratinocytes in
Coleman N, Stanley MA. Hum Pathol. 1994 Jan;25(1):73-9.