Immunofluorescent analysis of A431 cells (paraformaldehyde-fixed, 4%) labeling MRPL1 with ab180126 at 1/100 dilution followed by Goat anti rabbit IgG (Dylight 555) secondary at 1/250 dilution and counterstained with DAPI (blue).
All lanes : Anti-MRPL1 antibody [EPR14060-38] (ab180126) at 1/10000 dilutionLane 1 : MCF7 cell lysateLane 2 : Jurkat cell lysateLane 3 : HeLa cell lysateLane 4 : A431 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution