Anti-MRPL12 antibody
| Name | Anti-MRPL12 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab58334 |
| Prices | $390.00 |
| Sizes | 100 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Applications | WB IHC-P ICC/IF ICC/IF FC |
| Species Reactivities | Rat, Human |
| Antigen | Recombinant full length protein: MLPAAARPLW GPCLGLRAAA FRLARRQVPC VCAVRHMRSS GHQRCEALAG APLDNAPKEY PPKIQQLVQD IASLTLLEIS DLNELLKKTL KIQDVGLVPM GGVMSGAVPA AAAQEAVEED IPIAKERTHF TVRLTEAKPV DKVKLIKEIK NYIQGINLVQ AKKLVESLPQ EIKANVAKAE AEKIKAALEA VGGTVVLE, corresponding to amino acids 1-199 of Human MRPL12 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | MRPL12 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ICC/IF image of ab58334 stained CaCo2 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody ab58334 at 10µg/ml overnight at +4°C. The secondary antibody (green) was DyLight® 488 goat anti- mouse (ab96879) IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
MRPL12 antibody (ab58334) used in immunohistochemistry at 3ug/ml on formalin fixed and paraffin embedded human breast cancer tissue.
![Overlay histogram showing HepG2 cells stained with ab58334 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58334, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/24568_MRPL12-Primary-antibodies-ab58334-1.jpg)
Overlay histogram showing HepG2 cells stained with ab58334 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab58334, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Product References
MRM2 and MRM3 are involved in biogenesis of the large subunit of the - MRM2 and MRM3 are involved in biogenesis of the large subunit of the
Rorbach J, Boesch P, Gammage PA, Nicholls TJ, Pearce SF, Patel D, Hauser A, Perocchi F, Minczuk M. Mol Biol Cell. 2014 Sep 1;25(17):2542-55.
C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial - C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial
Rorbach J, Gammage PA, Minczuk M. Nucleic Acids Res. 2012 May;40(9):4097-109.
C7orf30 specifically associates with the large subunit of the mitochondrial - C7orf30 specifically associates with the large subunit of the mitochondrial
Wanschers BF, Szklarczyk R, Pajak A, van den Brand MA, Gloerich J, Rodenburg RJ, Lightowlers RN, Nijtmans LG, Huynen MA. Nucleic Acids Res. 2012 May;40(9):4040-51.
Chronic ethanol feeding causes depression of mitochondrial elongation factor Tu - Chronic ethanol feeding causes depression of mitochondrial elongation factor Tu
Weiser B, Gonye G, Sykora P, Crumm S, Cahill A. Am J Physiol Gastrointest Liver Physiol. 2011 May;300(5):G815-22. doi:
Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S - Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S
Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers ZM, Lightowlers RN. Biochem J. 2010 Sep 15;430(3):551-8.