Anti-MRPL3 antibody
| Name | Anti-MRPL3 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab39268 |
| Prices | $370.00 |
| Sizes | 100 µg |
| Host | Goat |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA WB |
| Species Reactivities | Human |
| Antigen | Synthetic peptide: NVCQPGAPSITFA , corresponding to C terminal amino acids 336-348 of Human MRPL3 Run BLAST with Run BLAST with |
| Description | Goat Polyclonal |
| Gene | MRPL3 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
ab39268 stained Caco-2 cells. The cells were 100% methamol fixed for 5 minutes at room temperature and then incubated in 1%BSA / 0.3M glycine in 0.1% PBS-Tween for 1hour at room temperature to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab39268 at 10µg/ml) overnight at +4°C. The secondary antibody (pseudo-colored green) was 150133 used at a 1/1000 dilution for 1hour at room temperature. Alexa Fluor® 594 WGA was used to label plasma membranes (pseudo-colored red) at a 1/200 dilution for 1hour at room temperature. DAPI was used to stain the cell nuclei (pseudo-colored blue) at a concentration of 1.43µM for 1hour at room temperature.
Product References
MRM2 and MRM3 are involved in biogenesis of the large subunit of the - MRM2 and MRM3 are involved in biogenesis of the large subunit of the
Rorbach J, Boesch P, Gammage PA, Nicholls TJ, Pearce SF, Patel D, Hauser A, Perocchi F, Minczuk M. Mol Biol Cell. 2014 Sep 1;25(17):2542-55.
C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial - C7orf30 is necessary for biogenesis of the large subunit of the mitochondrial
Rorbach J, Gammage PA, Minczuk M. Nucleic Acids Res. 2012 May;40(9):4097-109.
C7orf30 specifically associates with the large subunit of the mitochondrial - C7orf30 specifically associates with the large subunit of the mitochondrial
Wanschers BF, Szklarczyk R, Pajak A, van den Brand MA, Gloerich J, Rodenburg RJ, Lightowlers RN, Nijtmans LG, Huynen MA. Nucleic Acids Res. 2012 May;40(9):4040-51.
p32/gC1qR is indispensable for fetal development and mitochondrial translation: - p32/gC1qR is indispensable for fetal development and mitochondrial translation:
Yagi M, Uchiumi T, Takazaki S, Okuno B, Nomura M, Yoshida S, Kanki T, Kang D. Nucleic Acids Res. 2012 Oct;40(19):9717-37.
PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human - PDE12 removes mitochondrial RNA poly(A) tails and controls translation in human
Rorbach J, Nicholls TJ, Minczuk M. Nucleic Acids Res. 2011 Sep 1;39(17):7750-63.
Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes - Targeting of the cytosolic poly(A) binding protein PABPC1 to mitochondria causes
Wydro M, Bobrowicz A, Temperley RJ, Lightowlers RN, Chrzanowska-Lightowlers ZM. Nucleic Acids Res. 2010 Jun;38(11):3732-42.
Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S - Human ERAL1 is a mitochondrial RNA chaperone involved in the assembly of the 28S
Dennerlein S, Rozanska A, Wydro M, Chrzanowska-Lightowlers ZM, Lightowlers RN. Biochem J. 2010 Sep 15;430(3):551-8.