Anti-MRPL48 antibody [EPR16328]

• Supplier: Abcam
• Catalog: ab194826
• Prices: $357.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR16328
• Applications: IHC-P WB ICC/IF ICC/IF IP
• Species Reactivities: Mouse, Rat, Human
• Antigen: Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human MRPL48 aa 1-100
• Description: Rabbit Monoclonal
• Gene: MRPL48
• Conjugate: Unconjugated

Product images

All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilutionLane 1 : Mouse brain lysateLane 2 : Mouse heart lysateLane 3 : Rat brain lysateLane 4 : Rat heart lysateLane 5 : PC12 cell lysateLane 6 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling MRPL48 with ab194826 at 1/150 dilution. A Goat Anti-Rabbit IgG H&L (HRP) at 1/500 dilution was used as secondary (ab97051). Counterstain: Hematoxylin.Inset image: negative control obtained using PBS instead of ab194826. Cytoplasm staining on human cervix carcinoma tissue was observed.

Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/2000 dilution + Human fetal heart lysate at 20 µgSecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

All lanes : Anti-MRPL48 antibody [EPR16328] (ab194826) at 1/10000 dilutionLane 1 : HepG2 cell lysateLane 2 : HeLa cell lysateLane 3 : Jurkat cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution

Western blot analysis of immunoprecipitation pellet from HepG2 cell lysate immunoprecipitated using ab194826 at 1/30 dilution (lane 1) or PBS control (lane 2)For Western blot: ab194826 was used at 1/1000 dilution (0.6µg/ml). Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.

Immunofluorescent analysis of A431 cells labeling MRPL48 with ab194826 at 1/50 dilution. A Goat anti rabbit IgG (Alexa Fluor488) at 1/400 dilution (ab150077) was used as secondary antibody. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% triton X-100. Counterstain: DAPI. Cytoplasm staining on HepG2 cell line was observed.