Anti-MST4 antibody [EP1864Y]
| Name | Anti-MST4 antibody [EP1864Y] |
|---|---|
| Supplier | Abcam |
| Catalog | ab52491 |
| Prices | $313.00 |
| Sizes | 100 µl |
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | IgG |
| Clone | EP1864Y |
| Applications | WB IP ICC/IF FC IHC-P |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide corresponding to residues on the N terminus of human Mst4 |
| Description | Rabbit Monoclonal |
| Gene | STK26 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Overlay histogram showing HeLa cells stained with ab52491 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52491, 1/10000 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (0.1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
Immunohistochemical analysis of paraffin-embedded human placenta using ab52491 at a 1/100 dilution.
![Anti-MST4 antibody [EP1864Y] (ab52491) at 1/200000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25241_52491%2520WB.jpg)
Anti-MST4 antibody [EP1864Y] (ab52491) at 1/200000 dilution + HeLa cell lysate at 10 µgSecondaryGoat anti-rabbit HRP at 1/2000 dilution
Product References
Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation - Adaptor protein cerebral cavernous malformation 3 (CCM3) mediates phosphorylation
Fidalgo M, Guerrero A, Fraile M, Iglesias C, Pombo CM, Zalvide J. J Biol Chem. 2012 Mar 30;287(14):11556-65.
Merlin/ERM proteins establish cortical asymmetry and centrosome position. - Merlin/ERM proteins establish cortical asymmetry and centrosome position.
Hebert AM, DuBoff B, Casaletto JB, Gladden AB, McClatchey AI. Genes Dev. 2012 Dec 15;26(24):2709-23.
CCM3 signaling through sterile 20-like kinases plays an essential role during - CCM3 signaling through sterile 20-like kinases plays an essential role during
Zheng X, Xu C, Di Lorenzo A, Kleaveland B, Zou Z, Seiler C, Chen M, Cheng L, Xiao J, He J, Pack MA, Sessa WC, Kahn ML. J Clin Invest. 2010 Aug;120(8):2795-804.
Functional analyses of human and zebrafish 18-amino acid in-frame deletion pave - Functional analyses of human and zebrafish 18-amino acid in-frame deletion pave
Voss K, Stahl S, Hogan BM, Reinders J, Schleider E, Schulte-Merker S, Felbor U. Hum Mutat. 2009 Jun;30(6):1003-11.
Mst4 and Ezrin induce brush borders downstream of the Lkb1/Strad/Mo25 - Mst4 and Ezrin induce brush borders downstream of the Lkb1/Strad/Mo25
ten Klooster JP, Jansen M, Yuan J, Oorschot V, Begthel H, Di Giacomo V, Colland F, de Koning J, Maurice MM, Hornbeck P, Clevers H. Dev Cell. 2009 Apr;16(4):551-62.