Anti-MTA2 antibody
| Name | Anti-MTA2 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab8106 |
| Prices | $394.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | WB IHC-F IHC-P ICC/IF ICC/IF ELISA IP |
| Species Reactivities | Mouse, Rat, Human |
| Antigen | Synthetic peptide: PAPSHPASTNEPIVLED , corresponding to amino acids 652 - 668 of Human MTA 2 |
| Blocking Peptide | Human MTA2 peptide |
| Description | Rabbit Polyclonal |
| Gene | MTA2 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
All lanes : Anti-MTA2 antibody (ab8106) at 1 µg/mlLane 1 : HeLa whole cell lysate with absence of blocking peptideLane 2 : HeLa whole cell lysate with presence of blocking peptide
ab8106 at 10µg/ml staining MTA2 in Hela cells by ICC/IF
ab8106 (2µg/ml) staining MTA2 in human ileum using an automated system (DAKO Autostainer Plus). Using this protocol there is strong nuclear staining.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
![MTA2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MTA2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8106.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 77kDa: MTA2](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25329_MTA2-Primary-antibodies-ab8106-3.jpg)
MTA2 was immunoprecipitated using 0.5mg Hela whole cell extract, 5µg of Rabbit polyclonal to MTA2 and 50µl of protein G magnetic beads (+). No antibody was added to the control (-). The antibody was incubated under agitation with Protein G beads for 10min, Hela whole cell extract lysate diluted in RIPA buffer was added to each sample and incubated for a further 10min under agitation.Proteins were eluted by addition of 40µl SDS loading buffer and incubated for 10min at 70oC; 10µl of each sample was separated on a SDS PAGE gel, transferred to a nitrocellulose membrane, blocked with 5% BSA and probed with ab8106.Secondary: Mouse monoclonal [SB62a] Secondary Antibody to Rabbit IgG light chain (HRP) (ab99697).Band: 77kDa: MTA2
Immunofluorescence of PID in HeLa cells using ab8106 at 10 ug/ml.
Product References
An amino terminal phosphorylation motif regulates intranuclear - An amino terminal phosphorylation motif regulates intranuclear
Meijer DH, Sun Y, Liu T, Kane MF, Alberta JA, Adelmant G, Kupp R, Marto JA, Rowitch DH, Nakatani Y, Stiles CD, Mehta S. J Neurosci. 2014 Jun 18;34(25):8507-18.
Dual inhibition of EGFR at protein and activity level via combinatorial blocking - Dual inhibition of EGFR at protein and activity level via combinatorial blocking
Li J, Zhang L, Gao Z, Kang H, Rong G, Zhang X, Chen C. Protein Cell. 2014 Jun;5(6):457-68.
AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the - AGO2 and SETDB1 cooperate in promoter-targeted transcriptional silencing of the
Cho S, Park JS, Kang YK. Nucleic Acids Res. 2014 Dec 16;42(22):13545-56.
A direct physical interaction between Nanog and Sox2 regulates embryonic stem - A direct physical interaction between Nanog and Sox2 regulates embryonic stem
Gagliardi A, Mullin NP, Ying Tan Z, Colby D, Kousa AI, Halbritter F, Weiss JT, Felker A, Bezstarosti K, Favaro R, Demmers J, Nicolis SK, Tomlinson SR, Poot RA, Chambers I. EMBO J. 2013 Aug 14;32(16):2231-47.
An Oct4-centered protein interaction network in embryonic stem cells. - An Oct4-centered protein interaction network in embryonic stem cells.
van den Berg DL, Snoek T, Mullin NP, Yates A, Bezstarosti K, Demmers J, Chambers I, Poot RA. Cell Stem Cell. 2010 Apr 2;6(4):369-81.
Epigenetic differences in cortical neurons from a pair of monozygotic twins - Epigenetic differences in cortical neurons from a pair of monozygotic twins
Mastroeni D, McKee A, Grover A, Rogers J, Coleman PD. PLoS One. 2009 Aug 12;4(8):e6617.
Members of the NuRD chromatin remodeling complex interact with AUF1 in developing - Members of the NuRD chromatin remodeling complex interact with AUF1 in developing
Lee C, Gyorgy A, Maric D, Sadri N, Schneider RJ, Barker JL, Lawson M, Agoston DV. Cereb Cortex. 2008 Dec;18(12):2909-19.
SATB2 interacts with chromatin-remodeling molecules in differentiating cortical - SATB2 interacts with chromatin-remodeling molecules in differentiating cortical
Gyorgy AB, Szemes M, de Juan Romero C, Tarabykin V, Agoston DV. Eur J Neurosci. 2008 Feb;27(4):865-73.
Combinatorial effects of splice variants modulate function of Aiolos. - Combinatorial effects of splice variants modulate function of Aiolos.
Caballero R, Setien F, Lopez-Serra L, Boix-Chornet M, Fraga MF, Ropero S, Megias D, Alaminos M, Sanchez-Tapia EM, Montoya MC, Esteller M, Gonzalez-Sarmiento R, Ballestar E. J Cell Sci. 2007 Aug 1;120(Pt 15):2619-30.