Anti-MTHFD2 antibody - C-terminal (ab176016) at 1/1000 dilution + NCI-H460 cell line lysate at 35 µg
Immunohistochemical analysis of formalin-fixed and paraffin-embedded Human colon carcinoma tissue labelling MTHFD2 with ab176016 at 1/1000, followed by peroxidase conjugation to the secondary antibody and DAB staining.
Flow cytometric analysis of NCI-H460 cells labelling MTHFD2 with ab176016 at 1/10 dilution (bottom histogram), compared to a negative control cell (top histogram). FITC-conjugated Goat anti-Rabbit secondary antibodies were used for the analysis.