![Overlay histogram showing A549 cells stained with ab113637 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113637, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25545_ab113637-7-ab113637FC.jpg)
Overlay histogram showing A549 cells stained with ab113637 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab113637, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in A549 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
![Anti-MTHFR antibody [5D3] (ab113637) at 1/500 dilution + Rat heart cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25546_MTHFR-Primary-antibodies-ab113637-1.jpg)
Anti-MTHFR antibody [5D3] (ab113637) at 1/500 dilution + Rat heart cell lysate
![All lanes : Anti-MTHFR antibody [5D3] (ab113637) at 1/500 dilutionLane 1 : non transfected HEK293 cell lysate Lane 2 : MTHFR-hIgGFc transfected HEK293 cell lysate](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25547_MTHFR-Primary-antibodies-ab113637-2.jpg)
All lanes : Anti-MTHFR antibody [5D3] (ab113637) at 1/500 dilutionLane 1 : non transfected HEK293 cell lysate Lane 2 : MTHFR-hIgGFc transfected HEK293 cell lysate
Immunohistochemical analysis of paraffin-embedded human lung cancer using 1/200 ab113637 with DAB staining.
Immunohistochemical analysis of paraffin-embedded human intima cancer tissues using 1/200 ab113637 with DAB staining.
Immunohistochemical analysis of paraffin-embedded human prostate tissues using 1/200 ab113637 with DAB staining.
Elisa using ab113637