Anti-mTOR (phospho S2448) antibody [EPR426(2)]

• Supplier: Abcam
• Catalog: ab109268
• Prices: $392.00
• Sizes: 100 µl
• Host: Rabbit
• Clonality: Monoclonal
• Isotype: IgG
• Clone: EPR426(2)
• Applications: WB IHC-P ICC/IF ICC/IF FC
• Species Reactivities: Mouse, Rat, Human, Pig
• Antigen: Phospho specific peptide corresponding to residues surrounding Serine 2448 of Human mTOR
• Description: Rabbit Monoclonal
• Gene: MTOR
• Conjugate: Unconjugated

Product images

Overlay histogram showing HeLa cells stained with ab109268 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab109268, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) (ab150077) at 1/2000 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x106 cells) used under the same conditions. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 20mW Argon ion laser (488nm) and 525/30 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

All lanes : Anti-mTOR (phospho S2448) antibody [EPR426(2)] (ab109268) at 1/1000 dilutionLane 1 : 293T cell lysate, untreatedLane 2 : 293T cell lysate, treated with Alkaline Phosphatase Lysates/proteins at 10 µg per lane.

Immunohistochemical analysis of paraffin-embedded Human breast carcinoma tissue using ab109268 at a dilution of 1/50.

Immunofluorescent staining of HeLa cells using ab109268 at a dilution of 1/100.

Product References

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Duan Y, Fu W, Wang S, Ni Y, Zhao R. Comp Biochem Physiol A Mol Integr Physiol. 2014 Mar;169:90-5. doi:

Autophagic response in the Rabbit Hemorrhagic Disease, an animal model of - Autophagic response in the Rabbit Hemorrhagic Disease, an animal model of

Vallejo D, Crespo I, San-Miguel B, Alvarez M, Prieto J, Tunon MJ, Gonzalez-Gallego J. Vet Res. 2014 Feb 4;45:15.

Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and - Molecular switch role of Akt in Polygonatum odoratum lectin-induced apoptosis and

Li C, Chen J, Lu B, Shi Z, Wang H, Zhang B, Zhao K, Qi W, Bao J, Wang Y. PLoS One. 2014 Jul 3;9(7):e101526.

iASPP is a novel autophagy inhibitor in keratinocytes. - iASPP is a novel autophagy inhibitor in keratinocytes.

Chikh A, Sanza P, Raimondi C, Akinduro O, Warnes G, Chiorino G, Byrne C, Harwood CA, Bergamaschi D. J Cell Sci. 2014 Jul 15;127(Pt 14):3079-93.

Shikonin inhibits prostate cancer cells metastasis by reducing matrix - Shikonin inhibits prostate cancer cells metastasis by reducing matrix

Chen Y, Zheng L, Liu J, Zhou Z, Cao X, Lv X, Chen F. Int Immunopharmacol. 2014 Aug;21(2):447-55.

Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional - Progressive increase in mtDNA 3243A>G heteroplasmy causes abrupt transcriptional

Picard M, Zhang J, Hancock S, Derbeneva O, Golhar R, Golik P, O'Hearn S, Levy S, Potluri P, Lvova M, Davila A, Lin CS, Perin JC, Rappaport EF, Hakonarson H, Trounce IA, Procaccio V, Wallace DC. Proc Natl Acad Sci U S A. 2014 Sep 23;111(38):E4033-42. doi:

Butein Inhibits Angiogenesis of Human Endothelial Progenitor Cells via the - Butein Inhibits Angiogenesis of Human Endothelial Progenitor Cells via the

Chung CH, Chang CH, Chen SS, Wang HH, Yen JY, Hsiao CJ, Wu NL, Chen YL, Huang TF, Wang PC, Yeh HI, Wang SW. Evid Based Complement Alternat Med. 2013;2013:943187.

Akebia saponin PA induces autophagic and apoptotic cell death in AGS human - Akebia saponin PA induces autophagic and apoptotic cell death in AGS human

Xu MY, Lee DH, Joo EJ, Son KH, Kim YS. Food Chem Toxicol. 2013 Sep;59:703-8.

Phospho-mTOR: a novel target in regulation of renal lipid metabolism abnormality - Phospho-mTOR: a novel target in regulation of renal lipid metabolism abnormality

Hao J, Zhu L, Li F, Liu Q, Zhao X, Liu S, Xing L, Feng X, Duan H. Exp Cell Res. 2013 Aug 15;319(14):2296-306.

Leukemia inhibitory factor promotes nasopharyngeal carcinoma progression and - Leukemia inhibitory factor promotes nasopharyngeal carcinoma progression and

Liu SC, Tsang NM, Chiang WC, Chang KP, Hsueh C, Liang Y, Juang JL, Chow KP, Chang YS. J Clin Invest. 2013 Dec;123(12):5269-83.