Anti-mtRNA polymerase antibody

• Supplier: Abcam
• Catalog: ab32988
• Prices: $370.00
• Sizes: 200 µl
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: ICC/IF ICC/IF WB
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 1200 to the C-terminus of Human mtRNA polymerase
• Description: Rabbit Polyclonal
• Gene: POLRMT
• Conjugate: Unconjugated

Product images

All lanes : Anti-mtRNA polymerase antibody (ab32988) at 1/250 dilutionLane 1 : HeLa (Human epithelial carcinoma cell line) Whole Cell LysateLane 2 : HEK293 (Human embryonic kidney cell line) Whole Cell LysateLane 3 : Caco 2 (Human colonic carcinoma cell line) Whole Cell LysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

ICC/IF image of ab32988 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab32988, 1µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue). This antibody also gave a positive IF result in 4% PFA fixed MCF7 cells. However, this Fast-Track antibody is not yet fully characterised. This image represents inconclusive preliminary data.

Product References

The leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) does not - The leucine-rich pentatricopeptide repeat-containing protein (LRPPRC) does not

Harmel J, Ruzzenente B, Terzioglu M, Spahr H, Falkenberg M, Larsson NG. J Biol Chem. 2013 May 31;288(22):15510-9.

Negative regulation of mitochondrial transcription by mitochondrial topoisomerase - Negative regulation of mitochondrial transcription by mitochondrial topoisomerase

Sobek S, Dalla Rosa I, Pommier Y, Bornholz B, Kalfalah F, Zhang H, Wiesner RJ, von Kleist-Retzow JC, Hillebrand F, Schaal H, Mielke C, Christensen MO, Boege F. Nucleic Acids Res. 2013 Nov;41(21):9848-57.

Oxidative stress and mitochondrial functions in the intestinal Caco-2/15 cell - Oxidative stress and mitochondrial functions in the intestinal Caco-2/15 cell

Taha R, Seidman E, Mailhot G, Boudreau F, Gendron FP, Beaulieu JF, Menard D, Delvin E, Amre D, Levy E. PLoS One. 2010 Jul 27;5(7):e11817.