Anti-Mu Opioid Receptor antibody
| Name | Anti-Mu Opioid Receptor antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab10275 |
| Prices | $391.00 |
| Sizes | 50 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF WB IHC-F ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Guinea Pig, Human, Bovine, Pig, Primate, Monkey |
| Antigen | Synthetic peptide: NHQLENLEAETAPLP , corresponding to C terminal amino acids 384-398 of Rat Mu Opioid Receptor 1 |
| Blocking Peptide | Rat Mu Opioid Receptor peptide |
| Description | Rabbit Polyclonal |
| Gene | OPRM1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab10275 (1:2500) staining of Mu opioid receptor by IHC.
ICC/IF image of ab10275 stained SHSY5Y cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab10275, 1 in 200 dilution) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Immunohistochemical analysis of rat brain tissue, staining Mu Opioid Receptor with ab10275. Tissue was blocked with 5% normal donkey serum and 0.4% Triton X-100 for 1 hour at room temperature. Sections were incubated with primary antibody (1/2000) for 24 hours at 4°C. An AlexaFluor®594-conjugated donkey anti-rabbit IgG (1/250) was used as the secondary antibody.
Mu Opioid Receptor (ab10275) staining of Rat DRG (dilution: 1:100) incubation at 4 °C overnight. Secondary antibody is anti-Rabbit Rhodamine Red (dilution:1:200) incubation at room temperature for 1 hour.
Product References
TGF-beta and opioid receptor signaling crosstalk results in improvement of - TGF-beta and opioid receptor signaling crosstalk results in improvement of
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Morphine induces albuminuria by compromising podocyte integrity. - Morphine induces albuminuria by compromising podocyte integrity.
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mu-Opioid receptor antibody reveals tissue-dependent specific staining and - mu-Opioid receptor antibody reveals tissue-dependent specific staining and
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Repeated methamphetamine administration differentially alters fos expression in - Repeated methamphetamine administration differentially alters fos expression in
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Functional connectome of the striatal medium spiny neuron. - Functional connectome of the striatal medium spiny neuron.
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Muscarinic acetylcholine receptor modulation of mu (mu) opioid receptors in adult - Muscarinic acetylcholine receptor modulation of mu (mu) opioid receptors in adult
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Reduction of cell proliferation and potentiation of Fas-induced apoptosis by the - Reduction of cell proliferation and potentiation of Fas-induced apoptosis by the
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Immune cell-derived opioids protect against neuropathic pain in mice. - Immune cell-derived opioids protect against neuropathic pain in mice.
Labuz D, Schmidt Y, Schreiter A, Rittner HL, Mousa SA, Machelska H. J Clin Invest. 2009 Feb;119(2):278-86.
Involvement of Ca2+-dependent PKCs in the adaptive changes of mu-opioid pathways - Involvement of Ca2+-dependent PKCs in the adaptive changes of mu-opioid pathways
Giaroni C, Zanetti E, Pascale A, Oldrini R, Canciani L, Giuliani D, Amadio M, Chiaravalli AM, Lecchini S, Frigo GM. Biochem Pharmacol. 2009 Nov 1;78(9):1233-41.