ab124654 staining MUC13 in mouse colon tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with paraformaldehyde and blocked for 30 minutes at room temperature. Samples were incubated with primary antibody (1/25 in PBS Tween) for 1 hour. An undiluted HRP polymer-conjugated goat anti-rabbit IgG polyclonal was used as the secondary antibody.See Abreview
ab124654, at 5 µg/ml, staining MUC13 in Formalin-fixed, Paraffin-embedded Human Colon tissue by Immunohistochemistry.
All lanes : Anti-MUC13 antibody (ab124654) at 1/500 dilutionLane 1 : 293 cell lysateLane 2 : COLO205 cell lysateLane 3 : HeLa cell lysateLane 4 : HeLa cell lysate with synthesized peptide
ab124654 at 1/100 dilution staining MUC13 in HepG2 cells by Immunofluorescence. The image on the right is treated with the synthesized peptide.