Anti-MUC7 antibody
| Name | Anti-MUC7 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab55542 |
| Prices | $370.00 |
| Sizes | 50 µg |
| Host | Mouse |
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Applications | IHC-P ICC/IF ICC/IF WB FC |
| Species Reactivities | Human |
| Antigen | Recombinant fragment: HQSPKSHFEL PHYPGLLAHQ KPFIRKSYKC LHKRCRPKLP PSPNNPPKFP NPHQPPKHPD KNSSVVNPTL VATTQIPSVT FPSASTKITT LPNVTFLPQN , corresponding to amino acids 36-136 of Human MUC7 Run BLAST with Run BLAST with |
| Description | Mouse Monoclonal |
| Gene | MUC7 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Western blot against tagged recombinant protein immunogen using ab55542 MUC7 antibody at 1ug/ml.
ab55542 (1µg/ml) staining MUC7 in human salivary gland using an automated system (DAKO Autostainer Plus). Using this protocol there is strong cytoplasmic/secreted staining of glandular epithelium. Ductal epithelium is negative.Sections were rehydrated and antigen retrieved with the Dako 3 in 1 AR buffer citrate pH 6.0 in a DAKO PT link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 mins. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 min and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with Diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that, for manual staining, optimization of primary antibody concentration and incubation time is recommended. Signal amplification may be required.
ICC/IF image of ab55542 stained HeLa cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab55542, 5µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
![Overlay histogram showing MCF7 cells stained with ab55542 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55542, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.](http://www.bioprodhub.com/system/product_images/ab_products/2/sub_3/25959_MUC7-Primary-antibodies-ab55542-3.jpg)
Overlay histogram showing MCF7 cells stained with ab55542 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab55542, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2a [ICIGG2A] (ab91361, 1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in MCF7 cells fixed with 4% paraformaldehyde (10 min)/permeabilized in 0.1% PBS-Tween used under the same conditions.
Product References
Immunoglobulin A defines secretions from salivary tissue in post-Sistrunk - Immunoglobulin A defines secretions from salivary tissue in post-Sistrunk
Baig SM, Martinez-Alvernia EA, Mankarious LA. Otolaryngol Head Neck Surg. 2011 Jun;144(6):888-90. doi:
Cystic fibrosis and the relationship between mucin and chloride secretion by - Cystic fibrosis and the relationship between mucin and chloride secretion by
Finkbeiner WE, Zlock LT, Morikawa M, Lao AY, Dasari V, Widdicombe JH. Am J Physiol Lung Cell Mol Physiol. 2011 Oct;301(4):L402-14. doi:
Drainage post-thyroglossal duct remnant surgery: possible source and analysis. - Drainage post-thyroglossal duct remnant surgery: possible source and analysis.
Bakar SA, Martinez-Alvernia EA, Mankarious LA. Otolaryngol Head Neck Surg. 2009 Mar;140(3):343-7. doi: