All lanes : Anti-Myeloid zinc finger 1 antibody (ab64866) at 1/500 dilutionLane 1 : Extracts from 293 cells with no immunising peptideLane 2 : Extracts from K562 cells with no immunising peptideLane 3 : Extracts from K562 cells with immunising peptide at 5 µgLysates/proteins at 5 µg per lane.
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ab64866 at a dilution of 1/50 - 1/100. Left hand panel - no immunising peptide; right hand panel - immunising peptide.
ICC/IF image of ab64866 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab64866, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.