Anti-Myeloperoxidase antibody

• Supplier: Abcam
• Catalog: ab45977
• Prices: $400.00
• Sizes: 100 µg
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Applications: IHC-F ICC/IF ICC/IF WB IHC-P
• Species Reactivities: Human, Marmoset, Rat
• Antigen: Synthetic peptide conjugated to KLH derived from within residues 150 - 250 of Human Myeloperoxidase
• Description: Rabbit Polyclonal
• Gene: MPO
• Conjugate: Unconjugated

Product images

ICC/IF image of ab45977 stained HeLa cells. The cells were 4% PFA fixed (10 min), permabilised in 0.1% PBS-Tween (20 min) and incubated with the antibody (ab45977, 5µg/ml) for 1h at room temperature. 1%BSA / 10% normal goat serum / 0.3M glycine was used to block non-specific protein-protein interactions. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red). DAPI was used to stain the cell nuclei (blue).

ab45977 (1/50) staining Myeloperoxidase in paraffin-embbeded human spleen tissue sections. Tissue underwent fixation in formaldehyde, heat-mediated antigen retrieval in citrate buffer pH 6.0 and blocking (5 minutes/peroxidase block and 10 minutes/protein block). For further experimental details please refer to abreview.See Abreview

IHC image of 45977 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab45977, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Immunohistochemistical detection of Myeloperoxidase using antibody ab45977 on formaldehyde-fixed paraffin embedded Marmoset spleen sections.  Antigen retrieval step: heat mediated in citric acidpH6 HIER. Permeabilization: No Blocking step: 1% BSA for 10 mins @ rt°C. Primary antibody diluted @ 1/600 and incubated for 2 hours in TBS/BSA/azide. Secondary antibody: anti Rabbit IgG conjugated to biotin (1/200). Submitted image shows clear scattered cytoplasmic and (possibly secreted) positivity within the red pulp. Also, the blood vessel extending from upper middle to lower left of the image, shows clear, though lighter, granular positivity within the cytoplasm of 5 cells. Taken together, this is the pattern of positivity that I would expect.See Abreview

ab23106 staining p53 in Mouse bone marrow cells by Immunocytochemistry/ Immunoflourescence. Cells were fixed in formaldehyde and permeabilized in 0.1% Triton X-100 prior to blocking in 5% Goat serum for 2 hours at 25°C. The primary antibody was diluted 1/500 in PBS and incubated with the sample for 9 hours at 4°C. The secondary antibody was Alexa Fluor® 488-conjugated Goat anti-Rabbit polyclonal, diluted 1/500.Nuclei were counterstained blue with DAPI.See Abreview

Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/50000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.

Anti-Myeloperoxidase antibody (ab45977) at 1 µg/ml + HL60 (Human promyelocytic leukemia cell line) Whole Cell Lysate at 20 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

Product References

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