Anti-Myeloperoxidase antibody
| Name | Anti-Myeloperoxidase antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab9535 |
| Prices | $392.00 |
| Sizes | 500 µl |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P IHC-F ICC/IF ICC/IF IHC-F |
| Species Reactivities | Mouse, Rat, Human, Pig, Monkey |
| Antigen | Myeloperoxidase isolated from human granulocytes |
| Description | Rabbit Polyclonal |
| Gene | MPO |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Ab9535 staining of multinucleated cell (Blue) in human blood smear. Antibody was used at 1/200 for 1 hour at 21°C.See Abreview
Ab9535 staining human normal tonsil. Staining is localised to the cytoplasm and LysosomesLeft panel: with primary antibody at 4 ug/ml. Right panel: isotype control.Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 antigen retrieval buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be requ
ab9535 staining Myeloperoxidase in paraffin-embedded rat tissue sections by IHC-P (formaldehyde-fixed paraffin-embedded Immunohistochemistry). Tissue was fixed with formaldehyde and blocked with 20% serum for 1 hour at 24°C; antigen retrieval was by heat mediation in citrate buffer (pH6). Samples were incubated with primary antibody (1/50 in milken) for 1 hour ate 24°C. A Biotin-conjugated goat anti rabbt polyclonal (1/500) was used as secondary antibody.See Abreview
Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human B Cell Lymphoma tissue, staining Myeloperoxidase with ab9535.
Product References
Metabolically induced liver inflammation leads to NASH and differs from LPS- or - Metabolically induced liver inflammation leads to NASH and differs from LPS- or
Liang W, Lindeman JH, Menke AL, Koonen DP, Morrison M, Havekes LM, van den Hoek AM, Kleemann R. Lab Invest. 2014 May;94(5):491-502.
Combined blockade of angiotensin II type 1 receptor and activation of peroxisome - Combined blockade of angiotensin II type 1 receptor and activation of peroxisome
Nenicu A, Korbel C, Gu Y, Menger MD, Laschke MW. Hum Reprod. 2014 May;29(5):1011-24.
Fascin is regulated by slug, promotes progression of pancreatic cancer in mice, - Fascin is regulated by slug, promotes progression of pancreatic cancer in mice,
Li A, Morton JP, Ma Y, Karim SA, Zhou Y, Faller WJ, Woodham EF, Morris HT, Stevenson RP, Juin A, Jamieson NB, MacKay CJ, Carter CR, Leung HY, Yamashiro S, Blyth K, Sansom OJ, Machesky LM. Gastroenterology. 2014 May;146(5):1386-96.e1-17. doi:
A novel zebrafish model to provide mechanistic insights into the inflammatory - A novel zebrafish model to provide mechanistic insights into the inflammatory
Huang SY, Feng CW, Hung HC, Chakraborty C, Chen CH, Chen WF, Jean YH, Wang HM, Sung CS, Sun YM, Wu CY, Liu W, Hsiao CD, Wen ZH. PLoS One. 2014 Aug 20;9(8):e104414.
Giardia duodenalis infection reduces granulocyte infiltration in an in vivo model - Giardia duodenalis infection reduces granulocyte infiltration in an in vivo model
Cotton JA, Motta JP, Schenck LP, Hirota SA, Beck PL, Buret AG. PLoS One. 2014 Oct 7;9(10):e109087.
Intra-uterine microbial colonization and occurrence of endometritis in women with - Intra-uterine microbial colonization and occurrence of endometritis in women with
Khan KN, Fujishita A, Kitajima M, Hiraki K, Nakashima M, Masuzaki H. Hum Reprod. 2014 Nov;29(11):2446-56.
Cellular FLICE-like inhibitory protein secures intestinal epithelial cell - Cellular FLICE-like inhibitory protein secures intestinal epithelial cell
Wittkopf N, Gunther C, Martini E, He G, Amann K, He YW, Schuchmann M, Neurath MF, Becker C. Gastroenterology. 2013 Dec;145(6):1369-79.
TLR2 mediates Helicobacter pylori-induced tolerogenic immune response in mice. - TLR2 mediates Helicobacter pylori-induced tolerogenic immune response in mice.
Sun X, Zhang M, El-Zataari M, Owyang SY, Eaton KA, Liu M, Chang YM, Zou W, Kao JY. PLoS One. 2013 Sep 13;8(9):e74595.
Prokineticin 2 is an endangering mediator of cerebral ischemic injury. - Prokineticin 2 is an endangering mediator of cerebral ischemic injury.
Cheng MY, Lee AG, Culbertson C, Sun G, Talati RK, Manley NC, Li X, Zhao H, Lyons DM, Zhou QY, Steinberg GK, Sapolsky RM. Proc Natl Acad Sci U S A. 2012 Apr 3;109(14):5475-80. doi:
Chemokine-related gene expression in the brain following ischemic stroke: no role - Chemokine-related gene expression in the brain following ischemic stroke: no role
Brait VH, Rivera J, Broughton BR, Lee S, Drummond GR, Sobey CG. Brain Res. 2011 Feb 4;1372:169-79.