All lanes : Anti-MYL9 [EPR13012(2)] antibody (ab191393) at 1/1000 dilutionLane 1 : Human stomach lysateLane 2 : Human colon cancer lysateLane 3 : Human uterus lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.
All lanes : Anti-MYL9 [EPR13012(2)] antibody (ab191393) at 1/1000 dilutionLane 1 : C6 cell lysateLane 2 : PC12 cell lysateLane 3 : NIH 3T3 cell lysateLysates/proteins at 10 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Anti-MYL9 [EPR13012(2)] antibody (ab191393) at 1/5000 dilution + rat stomach lysate at 10 µgSecondaryGoat Anti-Rabbit IgG, (H+L),Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Rat colon tissue labeling MYL9 with ab191393 at 1/700 dilution followed by pre-diluted HRP Polymer for Rabbit IgG secondary antibody and counter-stained with Hematoxylin. Inset: Negative control: using PBS instead of primary antibody.
Western blot analysis of immunoprecipitation pellet from Human stomachlysate immunoprecipitated using ab191393 at 1/40 dilution (lane 1) or PBS control (lane 2).Secondary: Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1500 dilution.