Standard Curve for Myoglobin (Analyte: Myoglobin protein (ab96036)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [BDI927] to Myoglobin (ab19607) at 5µg/ml and Detector Antibody Rabbit polyclonal to Myoglobin (ab78441) at 0.5µg/ml.
All lanes : Anti-Myoglobin antibody (ab78441) at 0.5 µg/mlLane 1 : Skeletal Muscle (Human) Tissue Lysate - adult normal tissue (ab29330)Lane 2 : Heart (Human) Tissue Lysate - adult normal tissue (ab29431)Lysates/proteins at 5 µg per lane.SecondaryGoat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.
All lanes : Anti-Myoglobin antibody (ab78441) at 0.5 µg/mlLane 1 : Human Myoglobin full length protein (ab77876) at 0.1 µgLane 2 : Human Myoglobin full length protein (ab77876) at 0.01 µgSecondaryGoat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilutiondeveloped using the ECL techniquePerformed under reducing conditions.Exposure time : 2 minutes