ab47702 staining Myoglobin in cow skeletal muscle cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed by heat fixation and blocked with 5% serum for 30 minutes at 23°C. Samples were incubated with primary antibody (1/2000) for 1 hour at 23°C. An Alexa Fluor® 594-conjugated goat anti-mouse IgG polyclonal (1/1000) was used as the secondary antibody. Green - dystrophin, Blue - Hoechst.See Abreview
Anti-Myoglobin antibody [6H8B5] (ab47702) + Truncated Myoglobin recombinant protein (Tagged)
Ab47702 staining human Myoglobin in human skeletal muscle tissue, showing cytoplasmic localization, with DAB staining.