Anti-Myosin light chain (phospho S20) antibody
| Name | Anti-Myosin light chain (phospho S20) antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab2480 |
| Prices | $403.00 |
| Sizes | 50 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | ICC/IF ICC/IF ELISA WB IHC-F IP IHC-P |
| Species Reactivities | Mouse, Rat, Human, C. elegans, Bird, Mammalian |
| Antigen | Human Myosin Light Chain phospho peptide corresponding to a region of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH) - KKRPQRAT(pS)NVFAMFD (aa 12-27) |
| Description | Rabbit Polyclonal |
| Gene | MYL9 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
IHC-P of ab2480 at 2.5 µg/ml staining both vascular and myometrial smooth muscle cells of the uterus. The image shows localization of the antibody as the precipitated red signal, with a hematoxylin purple nuclear counterstain.
ab2480 staining Myosin light chain (phospho S20) in Xenopus laevis embryos by immunohistochemistry (PFA-perfusion fixed frozen tissue sections). The tissue sections were fixed in 2% TCA for 30 minutes and washed with PBST (PBS+0.3% TritonX 100) for 30 minutes. Samples were then blocked in 10% normal goat serum (NGS) for 1 hour at room temperature and incubated with the primary antibody. A Cy5 labelled Goat polyclonal to rabbit IgG was used as secondary.The arrows shows that depleting E-cadherin does not have any effect on the expression of apical pMLC.
Immunofluroescence analysis of Nicaragua cichlid (Hypsophrys nicaraguensis) keratocytes, staining Myosin light chain (phospho S20) (green) with ab2480. Cells were cultured on intermediate adhesion strength surfaces and extracted with 4% PEG and 1% Triton X-100 in cytoskeleton stabilizing buffer. Cells were blocked with PBS-BT for 5 minutes, and then incubated with primary antibody diluted in PBS-BT. Cells were then fixed with 4% formaldehyde in PBS for 10 minutes.
Product References
MYPT1 regulates contractility and microtubule acetylation to modulate integrin - MYPT1 regulates contractility and microtubule acetylation to modulate integrin
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Role of Rab11 in planar cell polarity and apical constriction during vertebrate - Role of Rab11 in planar cell polarity and apical constriction during vertebrate
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GEF-H1 functions in apical constriction and cell intercalations and is essential - GEF-H1 functions in apical constriction and cell intercalations and is essential
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RNAi-mediated stathmin suppression reduces lung metastasis in an orthotopic - RNAi-mediated stathmin suppression reduces lung metastasis in an orthotopic
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TSC1 controls distribution of actin fibers through its effect on function of Rho - TSC1 controls distribution of actin fibers through its effect on function of Rho
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Regulation of myosin light chain kinase during insulin-stimulated glucose uptake - Regulation of myosin light chain kinase during insulin-stimulated glucose uptake
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Lulu regulates Shroom-induced apical constriction during neural tube closure. - Lulu regulates Shroom-induced apical constriction during neural tube closure.
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Cardiac myosin isoforms exhibit differential rates of MgADP release and MgATP - Cardiac myosin isoforms exhibit differential rates of MgADP release and MgATP
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An adhesion-dependent switch between mechanisms that determine motile cell shape. - An adhesion-dependent switch between mechanisms that determine motile cell shape.
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