All lanes : Anti-N myc interactor antibody [EPR11065(2)] (ab183724) at 1/20000 dilutionLane 1 : K562 cell lysateLane 2 : HeLa cell lysateLane 3 : HepG2 cell lysateLysates/proteins at 20 µg per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Immunohistochemical analysis of paraffin-embedded Human tonsil tissue labeling N myc interactor with ab183724 at 1/50 dilution. The slide is counterstained with Hematoxylin.
Immunofluorescence analysis of acetone-fixed HeLa cells labeling N myc interactor with ab183724 at 1/100 dilution. Goat anti-rabbit IgG (Alexa Fluor® 488) at 1/200 dilution was used as the secondary antibody (green). The slide on the right is stained with Dapi (blue).
Western blot analysis of K562 cell lysate precipitated with ab183724 at 1/50 dilution. A Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated secondary antibody at 1/1000 dilution was then used. The blocking buffer and dilution buffer was 5% NFDM/TBST.