All lanes : Anti-NAIP antibody (ab2549) at 1/1000 dilutionLane 1 : Lysate prepared from transfected PC3 cells.Lane 2 : Lysate prepared from non-transfected PC3 cells.Lane 3 : Lysate prepared from transfected HeLa cells.Lane 4 : Lysate prepared from non-transfected HeLa cells.Lane 5 : Lysate prepared from non-transfected NTera2D1 cells.Secondarysecondary antibody at 1/100000 dilutionObserved band size : 160 kDa (why is the actual band size different from the predicted?)Additional bands at : 105 kDa (possible isoform),115 kDa,130 kDa (possible isoform). We are unsure as to the identity of these extra bands.Image from Dr MT Romanish et al, PLoS One. 2009 Jun 2;4(6):e5761, Fig 5.