Anti-NAP1L1 antibody
| Name | Anti-NAP1L1 antibody |
|---|---|
| Supplier | Abcam |
| Catalog | ab21630 |
| Prices | $385.00 |
| Sizes | 100 µg |
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Applications | IHC-P WB ICC/IF ICC/IF |
| Species Reactivities | Human, Mouse, Rat, Dog, Xenopus |
| Antigen | Synthetic peptide conjugated to KLH derived from within residues 350 to the C-terminus of Human NAP1L1 |
| Blocking Peptide | Human NAP1L1 peptide |
| Description | Rabbit Polyclonal |
| Gene | NAP1L1 |
| Conjugate | Unconjugated |
| Supplier Page | Shop |
Product images
Lanes 1 - 2 : Anti-NAP1L1 antibody (ab21630) at 1/1000 dilutionLanes 3 - 4 : Anti-NAP1L1 antibody (ab21630) at 1/500 dilutionLanes 5 - 6 : Anti-NAP1L1 antibody (ab21630) at 1/250 dilutionLane 1 : U2OS cell lysateLane 2 : U2OS cell lysate treated with ionizing radiationLane 3 : U2OS cell lysateLane 4 : U2OS cell lysate treated with ionizing radiationLane 5 : U2OS cell lysateLane 6 : U2OS cell lysate treated with ionizing radiationPerformed under reducing conditions.
All lanes : Anti-NAP1L1 antibody (ab21630) at 1 µg/mlLane 1 : HeLa whole cell lysate at 20 µgLane 2 : A431 whole cell lysate at 20 µgLane 3 : HEK293 whole cell lysate at 20 µgLane 4 : HeLa whole cell lysate at 20 µg with Human NAP1L1 peptide (ab22417) at 1 µg/mlLane 5 : A431 whole cell lysate with Human NAP1L1 peptide (ab22417) at 1 µg/mlLane 6 : HEK293 whole cell lysate at 20 µg with Human NAP1L1 peptide (ab22417) at 1 µg/mlSecondaryAlexa fluor goat polyclonal to rabbit IgG at 1/10000 dilution
IHC image of ab21630 staining in human skin formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab21630, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
ICC/IF image of ab21630 stained A431 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab21630, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
Product References
The U4/U6 recycling factor SART3 has histone chaperone activity and associates - The U4/U6 recycling factor SART3 has histone chaperone activity and associates
Long L, Thelen JP, Furgason M, Haj-Yahya M, Brik A, Cheng D, Peng J, Yao T. J Biol Chem. 2014 Mar 28;289(13):8916-30.
Locus-specific proteomics by TChP: targeted chromatin purification. - Locus-specific proteomics by TChP: targeted chromatin purification.
Pourfarzad F, Aghajanirefah A, de Boer E, Ten Have S, Bryn van Dijk T, Kheradmandkia S, Stadhouders R, Thongjuea S, Soler E, Gillemans N, von Lindern M, Demmers J, Philipsen S, Grosveld F. Cell Rep. 2013 Aug 15;4(3):589-600.