All lanes : Anti-Nardilysin antibody - Aminoterminal end (ab46764) at 1/1000 dilutionLane 1 : Whole cell lysate from human testis-derived Hs1.Tes cells (treated with SF) Lane 2 : Whole cell lysate from human testis-derived Hs1.Tes cells (treated with TPA) Lane 3 : Whole cell lysate from human testis-derived Hs1.Tes cells (treated with IL1-beta) Lane 4 : Whole cell lysate from human testis-derived Hs1.Tes cells (treated with TNF-alpha) Lane 5 : Whole cell lysate from human testis-derived Hs1.Tes cells (treated with RA)